Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc Dulbecco's Phosphate Buffered Saline, DPBS - 20L-CBY
Dulbecco s Phosphate Buffered Saline DPBS - 20L-CBY (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Tris Buffered Saline, TBS (10X, pH 7.6) - 1L
Tris Buffered Saline TBS (10X pH 7 6) - 1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc CHAPS Lysis Buffer - 50ml
CHAPS Lysis Buffer - 50ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc HBS-P+ Buffer (10X, pH 7.4) - 4x500ml
HBS-P Plus Buffer (10X pH 7 4) - 4x500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc PIPES Buffer (1 M, pH 6.8) - 500ml
PIPES Buffer (1 M pH 6 8) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (10X, pH 7.4) - 500ml
Phosphate Buffered Saline PBS (10X pH 7 4) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (Sterile-Filtered, RNase Free) - 500ml
Phosphate Buffered Saline PBS (Sterile-Filtered RNase Free) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 6.8) - 500ml
Tris-HCl Buffer (1 M pH 6 8) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Tris-Base (1 M) - 2x1L
Bioprocess Grade - Tris-Base (1 M) - 2x1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Krebs-Henseleit Buffer (with HEPES, without Calcium) - 500ml
Krebs-Henseleit Buffer (with HEPES without Calcium) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Bovine Serum Albumin, BSA (5%, TBST) - 250ml
Bovine Serum Albumin BSA (5% TBST) - 250ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Tris-Glycine-Native Running Buffer (10X) - 1L
Tris-Glycine-Native Running Buffer (10X) - 1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc HEPES Buffered Saline (2X, Low Endotoxin) - 250ml
HEPES Buffered Saline (2X Low Endotoxin) - 250ml (For Research Use and Further Manufacturing Only)
Fd Neurotechnologies Inc PHOSPH-BUF .04PARAFORM W/ PA
Phosphate-Buffered 0.04 Paraformaldehyde with Picric Acid
Newcomer Supply Phosphate Buffer 0.1M, pH 7.2 to 7.4, 1 gallon
Use: IHC wash and rinse. Shelf Life is 1 year from date of manufacture. Store at 2-8°C.